Disney's boss has told employees who are currently working from home to start going into the office for four days a week from the start of March.
Chief executive Bob Iger said so-called "hybrid" workers will now be asked to treat "Monday through Thursday as in-person workdays".
He also highlighted his view that face-to-face collaboration is key to "a creative business like ours".
The announcement comes two months after Mr Iger's shock return to Disney.
"Nothing can replace the ability to connect, observe, and create with peers that comes from being physically together, nor the opportunity to grow professionally by learning from leaders and mentors, Mr Iger said in a memo to employees seen by the BBC.
"It is my belief that working together more in-person will benefit the company's creativity, culture, and our employees' careers," he added.