CRISPR-Cascade test detects bloodstream infections in minutes without amplification

This article has been reviewed according to Science X's editorial process and policies . Editors have highlighted the following attributes while ensuring the content's credibility: University of Illinois at Urbana-Champaign researchers have developed a CRISPR-based diagnostic tool capable of detecting bloodstream infections in minutes without the need for nucleic acid amplification. The CRISPR-Cascade assay achieves attomolar sensitivity and incorporates an OR-gated logic function to identify multiple pathogens simultaneously through DNA from pathogens associated with bloodstream infections. Bloodstream infections require rapid identification to prevent complications, yet standard diagnostic methods rely on...